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MagFinder_.py
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MagFinder_.py
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"""
Top-left of pixel is (0 , 0 )
Center of pixel is (0.5, 0.5)
"""
from __future__ import with_statement
import itertools
import os
import sys
import threading
import time
from contextlib import contextmanager
import ConfigParser
import ij
import jarray
import java
from fiji.util.gui import GenericDialogPlus
from ij import IJ, ImagePlus, ImageStack, WindowManager
from ij.gui import GenericDialog, OvalRoi, PointRoi, PolygonRoi, Roi
from ij.io import DirectoryChooser
from ij.plugin import MontageMaker
from ij.plugin.frame import RoiManager
from Jama import Matrix
from java.awt import Button, Checkbox, Color, Rectangle
from java.awt.event import ActionListener, KeyAdapter, KeyEvent, MouseAdapter
from java.awt.geom.Point2D import Float as pFloat
from java.io import File, FileInputStream
from java.lang import Exception as java_exception
from java.lang import Math, Runtime, System
from java.lang.reflect import Array
from java.net import URL
from java.nio.file import Files, Paths
from java.util import HashSet
from java.util.zip import GZIPInputStream
from mpicbg.models import Point, PointMatch, RigidModel2D
from net.imglib2.img import ImagePlusAdapter
from net.imglib2.img.display.imagej import ImageJFunctions as IL
from net.imglib2.interpolation.randomaccess import (
NearestNeighborInterpolatorFactory,
NLinearInterpolatorFactory,
)
from net.imglib2.realtransform import AffineTransform2D
from net.imglib2.realtransform import RealViews as RV
from net.imglib2.view import Views
from org.apache.commons.io import FileUtils
sys.path.append(IJ.getDirectory("plugins"))
from geometrycalculator import GeometryCalculator
from magreorderer import MagReorderer
DEBUG = False
SIZE_HANDLE = 15
LOCAL_SIZE_STANDARD = 400 # for local summary
# DISPLAY_FACTOR = 2.5
DISPLAY_FACTOR = 1.2
MSG_DRAWN_ROI_MISSING = (
"Please draw something before pressing [a]."
+ "\nAfter closing this message you can press [h] for help."
)
ACCEPTED_IMAGE_FORMATS = (
".tif",
".tiff",
".png",
".jpg",
".jpeg",
)
# these transforms are applied to annotations of lengths 1,2
# because they are displayed by Fiji with a (0.5,0.5) offset
# because they follow a different subpixel convention
# we pre-apply TRANSFORM_DISPLAY when going from wafer -> manager
TRANSFORM_DISPLAY = AffineTransform2D()
TRANSFORM_DISPLAY.translate([-0.5, -0.5])
# we pre-apply TRANSFORM_DISPLAY_INVERSE when going from manager -> wafer
TRANSFORM_DISPLAY_INVERSE = TRANSFORM_DISPLAY.inverse()
# these transforms fix the discrepancy between
# the Fiji ROI convention: top-left corner of pixel
# the Imglib2 convention: center of pixel
TRANSFORM_SUBPIXEL = AffineTransform2D()
TRANSFORM_SUBPIXEL.translate([0.5, 0.5])
TRANSFORM_SUBPIXEL_INVERSE = TRANSFORM_SUBPIXEL.inverse()
def mkdir_p(path):
path = os.path.join(path, "")
try:
os.mkdir(path)
IJ.log("Folder created: " + path)
except Exception as e:
if e[0] == 20047:
pass
else:
IJ.log("Exception during folder creation :" + str(e))
return path
def dlog(x):
"""Double log to print and dlog"""
IJ.log(x)
print(x)
def intr(x):
"""Float to int with rounding (instead of int(x) that does a floor)"""
return int(round(x))
def pairwise(iterable):
"""from https://docs.python.org/3/library/itertools.html#itertools.pairwise
'ABCD' --> AB BC CD"""
a, b = itertools.tee(iterable)
next(b, None)
return zip(a, b)
def get_square_row_col(n):
"""Returns a grid close to being square with n_rows*n_cols > n"""
n_rows = int(n**0.5)
n_cols = n // n_rows
if n_rows * n_cols < n:
n_rows += 1
return n_rows, n_cols
class Safety(object):
"""Safety level for shortcuts with user interaction"""
STRICT = "strict"
RELAXED = "relaxed"
class Neighbor(object):
PREVIOUS = "previous"
NEXT = "next"
class Direction(object):
"""Enum for directions"""
LEFT = "left"
RIGHT = "right"
UP = "up"
DOWN = "down"
class Mode(object):
"""Enum for the display modes"""
GLOBAL = "global"
LOCAL = "local"
class AnnotationTypeDef(object):
"""Parameters associated to an annotation type"""
def __init__(self, name, string, color, handle_size_global, handle_size_local):
self.name = name
self.string = string
self.color = color
self.handle_size_global = handle_size_global
self.handle_size_local = handle_size_local
class AnnotationType(object):
SECTION = AnnotationTypeDef("sections", "section", Color.blue, 15, 15)
ROI = AnnotationTypeDef("rois", "roi", Color.yellow, 15, 2)
FOCUS = AnnotationTypeDef("focus", "focus", Color.green, 15, 15)
MAGNET = AnnotationTypeDef("magnets", "magnet", Color.green, 15, 15)
LANDMARK = AnnotationTypeDef("landmarks", "landmark", Color.yellow, 15, 15)
@classmethod
def all(cls):
"""Returns all annotation types"""
return [cls.SECTION, cls.ROI, cls.FOCUS, cls.MAGNET, cls.LANDMARK]
@classmethod
def all_but_landmark(cls):
"""Returns all annotation types except landmarks"""
return [cls.SECTION, cls.ROI, cls.FOCUS, cls.MAGNET]
@classmethod
def section_annotations(cls):
"""Returns all annotation types except landmarks"""
return [cls.ROI, cls.FOCUS, cls.MAGNET]
class Wafer(object):
def __init__(self, magc_path=None):
self.mode = Mode.GLOBAL
self.root, self.image_path = self.init_image_path(magc_path)
self.tsp_solver = TSPSolver(self.root)
self.manager = init_manager()
if magc_path is None:
self.magc_path = self.init_magc_path()
else:
self.magc_path = magc_path
self.init_images_global()
self.sections = {}
self.rois = {}
self.focus = {}
self.magnets = {}
self.landmarks = {}
self.transforms = {}
self.poly_transforms = {}
"""poly_tranforms: global to local"""
self.poly_transforms_inverse = {}
"""poly_tranforms_inverse: local to global"""
self.serial_order = []
"""the serial order is the order in which the sections have been cut"""
self.stage_order = []
"""the stage_order is the order that minimizes microscope stage travel
to image one section after the other"""
self.GC = GeometryCalculator
self.file_to_wafer()
IJ.setTool("polygon")
def __len__(self):
"""Returns the number of sections"""
if not hasattr(self, "sections"):
return 0
return len(self.sections)
# TODO this image vs img does not look optimal
@property
def image(self):
if self.mode is Mode.GLOBAL:
return self.image_global
return self.image_local
@property
def img(self):
if self.mode is Mode.GLOBAL:
return self.image_global
return self.img_local
@property
def landmarks_xy(self):
return [self.landmarks[key].centroid for key in sorted(self.landmarks)]
@contextmanager
def set_mode(self, mode):
old_mode = self.mode
self.mode = mode
try:
yield
finally:
self.mode = old_mode
def set_listeners(self):
"""Sets key and mouse wheel listeners"""
add_key_listener_everywhere(KeyListener(self))
add_mouse_wheel_listener_everywhere(MouseWheelListener(self))
def set_global_mode(self):
"""useful when wafer accessed from another module"""
self.mode = Mode.GLOBAL
def set_local_mode(self):
self.mode = Mode.LOCAL
def init_image_path(self, magc_path=None):
"""
Finds the image used for navigation in magfinder.
It is the image with the smallest size in the directory
that does not contain "overview" in its name.
"""
if magc_path is not None:
root = os.path.dirname(magc_path)
else:
try:
root = os.path.normpath(
DirectoryChooser("Select the experiment folder.").getDirectory()
)
except Exception:
IJ.showMessage("Exit", "There was a problem accessing the folder")
sys.exit("No directory was selected. Exiting.")
if not os.path.isdir(root):
IJ.showMessage("Exit", "No directory was selected. Exiting.")
sys.exit("No directory was selected. Exiting.")
try:
wafer_im_path = sorted(
[
os.path.join(root, name)
for name in os.listdir(root)
if all(
(
any(name.endswith(x) for x in ACCEPTED_IMAGE_FORMATS),
"verview" not in name,
"annotated" not in name,
)
)
],
key=os.path.getsize, # the smallest image is the one used for magfinder navigation
)[0]
# )[1]
except IndexError:
IJ.showMessage(
"Message",
"There is no image (.tif, .png, .jpg, .jpeg, .tiff) in the experiment folder you selected."
"\nAdd an image and start again the plugin"
"\n(Disregard this message if you are doing a wafer transfer and know what you are doing)",
)
# sys.exit()
return root, None
return root, wafer_im_path
def init_magc_path(self):
"""Loads existing .magc file or creates a new one if does not exist"""
magc_paths = [
os.path.join(self.root, filename)
for filename in os.listdir(self.root)
if filename.endswith(".magc")
]
if not magc_paths:
dlog("No .magc file found. Creating an empty one")
wafer_name_from_user = get_name(
"Please name this substrate",
default_name="default_substrate",
cancel_msg="The substrate needs a name. Using 'default_substrate'.",
)
if not wafer_name_from_user:
wafer_name_from_user = "default_substrate"
magc_path = os.path.join(self.root, "{}.magc".format(wafer_name_from_user))
with open(magc_path, "w"):
pass
else:
magc_path = magc_paths[0]
return magc_path
def file_to_wafer(self):
"""Populates the wafer instance from the .magc file"""
config = ConfigParser.ConfigParser()
with open(self.magc_path, "rb") as configfile:
config.readfp(configfile)
for header in config.sections():
if "." in header:
annotation_type, section_id, annotation_id = type_id(
header, delimiter_0="."
)
for key, val in config.items(header):
if key in ["polygon", "location"]:
vals = [float(x) for x in val.split(",")]
points = [[x, y] for x, y in zip(vals[::2], vals[1::2])]
self.add(
annotation_type,
self.GC.points_to_poly(points),
(
(section_id, annotation_id)
if annotation_type is AnnotationType.ROI
else section_id
),
)
elif header in ["serial_order", "stage_order"]:
if config.get(header, header) != "[]":
setattr(
self,
header,
[int(x) for x in config.get(header, header).split(",")],
)
if not self.serial_order:
self.serial_order = sorted(self.sections.keys())
if not self.stage_order:
self.stage_order = sorted(self.sections.keys())
dlog(
(
"File successfully read with \n{} sections \n{} rois \n{} focus"
+ "\n{} magnets \n{} landmarks"
).format(
len(self),
len(self.rois),
len(self.focus),
len(self.magnets),
len(self.landmarks),
)
)
def manager_to_wafer(self):
"""
Populates the wafer from the roi manager
Typically called after the user interacted with the UI
"""
self.clear_annotations()
for roi in self.manager.iterator():
annotation_type, section_id, annotation_id = type_id(roi.getName())
# if self.mode is Mode.GLOBAL and roi.size() <= 2:
if roi.size() <= 2:
roi = GeometryCalculator.transform_poly(
poly=roi, aff=TRANSFORM_DISPLAY_INVERSE
)
self.add(
annotation_type,
roi,
(
(section_id, annotation_id)
if annotation_type is AnnotationType.ROI
else section_id
),
)
self.clear_transforms()
self.compute_transforms()
def wafer_to_manager(self):
"""Draws all rois from the wafer instance into the manager"""
self.manager.reset()
if self.mode is Mode.GLOBAL:
self.wafer_to_manager_global()
else:
self.wafer_to_manager_local()
def wafer_to_manager_global(self):
for landmark in self.landmarks.values():
if not (
0 < landmark.centroid[0] < self.image.getWidth()
and 0 < landmark.centroid[1] < self.image.getHeight()
):
# do not draw landmark if it is not visible,
# otherwise it creates a weird offset with negative coordinates
continue
self.manager.addRoi(landmark.poly_display)
landmark.poly.setHandleSize(landmark.type_.handle_size_global)
for section_id, section in sorted(self.sections.iteritems()):
self.manager.addRoi(section.poly_display)
section.poly.setHandleSize(section.type_.handle_size_global)
for annotation_type in [
AnnotationType.FOCUS,
AnnotationType.MAGNET,
]:
annotation = getattr(self, annotation_type.name).get(section_id)
if annotation is None:
continue
self.manager.addRoi(annotation.poly_display)
annotation.poly.setHandleSize(annotation_type.handle_size_global)
if section_id in self.rois:
for _, subroi in sorted(self.rois[section_id].iteritems()):
self.manager.addRoi(subroi.poly_display)
subroi.poly.setHandleSize(annotation_type.handle_size_global)
def wafer_to_manager_local(self):
for id_order, section_id in enumerate(self.serial_order):
for annotation_type in [
AnnotationType.SECTION,
AnnotationType.FOCUS,
AnnotationType.MAGNET,
]:
annotation = getattr(self, annotation_type.name).get(section_id)
if annotation is None:
continue
transform_poly_display = AffineTransform2D()
transform_poly_display.preConcatenate(self.poly_transforms[section_id])
transform_poly_display.preConcatenate(TRANSFORM_DISPLAY)
if len(annotation) <= 2:
transform_poly = transform_poly_display
else:
transform_poly = self.poly_transforms[section_id]
local_poly = self.GC.transform_points_to_poly(
annotation.points, transform_poly
)
local_poly.setName(str(annotation))
local_poly.setStrokeColor(annotation_type.color)
local_poly.setImage(self.image)
local_poly.setPosition(0, id_order + 1, 0)
local_poly.setHandleSize(annotation_type.handle_size_local)
self.manager.addRoi(local_poly)
# poly = self.GC.transform_points_to_poly(
# [annotation.centroid], self.poly_transforms[section_id]
# )
# if DEBUG:
# IJ.log(
# "{} | local {} {}".format(
# repr(annotation),
# poly.getFloatPolygon().xpoints,
# poly.getFloatPolygon().ypoints,
# )
# )
if section_id not in self.rois:
continue
for _, subroi in sorted(self.rois[section_id].iteritems()):
if len(subroi) <= 2:
transform_poly = transform_poly_display
else:
transform_poly = self.poly_transforms[section_id]
local_poly = self.GC.transform_points_to_poly(
subroi.points, transform_poly
)
local_poly.setName(str(subroi))
local_poly.setStrokeColor(subroi.type_.color)
local_poly.setImage(self.image)
local_poly.setPosition(0, id_order + 1, 0)
local_poly.setHandleSize(subroi.type_.handle_size_local)
self.manager.addRoi(local_poly)
# poly = self.GC.transform_points_to_poly(
# [subroi.centroid], self.poly_transforms[section_id]
# )
# if DEBUG:
# IJ.log(
# "{} | local {} {}".format(
# repr(subroi),
# poly.getFloatPolygon().xpoints,
# poly.getFloatPolygon().ypoints,
# )
# )
def clear_annotations(self):
"""Clears all annotations except the landmarks"""
self.sections = {}
self.rois = {}
self.focus = {}
self.magnets = {}
def clear_transforms(self):
"""Clears all transforms"""
self.transforms = {}
self.poly_transforms = {}
self.poly_transforms_inverse = {}
def save(self):
"""Saves the wafer annotations to the .magc file"""
dlog("Saving ...")
self.manager_to_wafer()
config = ConfigParser.ConfigParser()
for annotation_type in AnnotationType.all():
if annotation_type is AnnotationType.ROI:
continue
annotations = getattr(self, annotation_type.name)
if not annotations:
continue
config.add_section(annotation_type.name)
config.set(
annotation_type.name,
"number",
str(len(annotations)),
)
for _, annotation in sorted(annotations.iteritems()):
header = annotation.header
config.add_section(header)
if annotation_type in [
AnnotationType.SECTION,
AnnotationType.ROI,
AnnotationType.FOCUS,
]:
config.set(
header,
"polygon",
self.GC.points_to_flat_string(annotation.points),
)
if annotation_type in [
AnnotationType.SECTION,
AnnotationType.ROI,
]:
config.set(
header,
"center",
self.GC.point_to_flat_string(annotation.centroid),
)
config.set(header, "area", str(annotation.area))
config.set(
header,
"angle",
str(((annotation.angle - 90) % 360) - 180),
)
elif annotation_type in [
AnnotationType.MAGNET,
AnnotationType.LANDMARK,
]:
config.set(
header,
"location",
self.GC.point_to_flat_string(annotation.centroid),
)
config.add_section("end_{}".format(annotation_type.name))
if self.rois:
config.add_section(AnnotationType.ROI.name)
config.set(
AnnotationType.ROI.name,
"number",
sum([len(section_rois) for section_rois in self.rois.values()]),
)
for _, rois in sorted(self.rois.iteritems()):
for _, roi in sorted(rois.iteritems()):
header = roi.header
config.add_section(header)
config.set(
header,
"polygon",
self.GC.points_to_flat_string(roi.points),
)
config.set(
header,
"center",
self.GC.point_to_flat_string(roi.centroid),
)
config.set(header, "area", str(roi.area))
config.set(
header,
"angle",
str(((roi.angle - 90) % 360) - 180),
)
for order_name in ["serial_order", "stage_order"]:
config.add_section(order_name)
order = getattr(self, order_name)
if not order:
dlog("Debug: this case should not happen".center(100, "-"))
config.set(order_name, order_name, "[]")
else:
config.set(order_name, order_name, ",".join([str(x) for x in order]))
with open(self.magc_path, "w") as configfile:
config.write(configfile)
dlog("Saved to {}".format(self.magc_path))
# self.save_csv()
def close_mode(self):
"""
1.Saves the current wafer
2.Closes the current display mode
3.Restores the standard GUI key listeners
"""
self.save()
if self.mode is Mode.GLOBAL:
self.image.hide()
else:
self.image.close()
self.manager.reset()
# restore the keylisteners of the IJ window
map(IJ.getInstance().addKeyListener, initial_ij_key_listeners)
def start_local_mode(self):
"""Starts local display mode"""
dlog("Starting local mode ...")
self.mode = Mode.LOCAL
self.compute_transforms()
self.create_local_stack()
self.wafer_to_manager()
self.set_listeners()
self.manager.runCommand("UseNames", "false")
self.manager.runCommand("Show None")
set_roi_and_update_roi_manager(0) # select first ROI
self.arrange_windows()
def compute_transforms(self):
"""
1.self.transforms[section_key] transforms the global wafer image
to an image in which the section section_key is centered at 0
and has an angle of 0 degrees
2.the transform_poly is almost like the self.transforms except that
a. it contains an offset due to the fact that an ImagePlus is displayed
with their top-left corner at 0,0 and not at -w/2,-h/2
b. it does not contain the 0.5 offset change of basis to account for the fact
that ImageJ ROIs use top-left pixel corner vs pixel center from ImgLib2 transforms
"""
_, _, display_size, _ = self.get_display_parameters()
self.local_display_size = display_size
for section_id, section in self.sections.iteritems():
# transform for the image
transform_section_bare = AffineTransform2D()
transform_section_bare.translate([-v for v in section.centroid])
transform_section_bare.rotate(section.angle * Math.PI / 180.0)
self.transforms[section_id] = self.GC.change_basis(
A=transform_section_bare,
B=TRANSFORM_SUBPIXEL,
B_inverse=TRANSFORM_SUBPIXEL_INVERSE,
)
# transform for the poly
translation_poly = AffineTransform2D()
translation_poly.translate(
[
0.5 * self.local_display_size[0],
0.5 * self.local_display_size[1],
]
)
transform_poly = transform_section_bare.copy()
transform_poly.preConcatenate(translation_poly)
self.poly_transforms[section_id] = transform_poly
self.poly_transforms_inverse[section_id] = transform_poly.inverse()
def create_local_stack(self, straight_transforms=None):
"""Creates the local stack with imglib2 framework"""
transforms = (
self.transforms if straight_transforms is None else straight_transforms
)
display_params = (
[
int(-0.5 * self.local_display_size[0]),
int(-0.5 * self.local_display_size[1]),
],
[
int(0.5 * self.local_display_size[0]),
int(0.5 * self.local_display_size[1]),
],
)
if DEBUG:
IJ.log("local stack {}".format(display_params))
imgs = [
Views.interval(
RV.transform(
Views.interpolate(
Views.extendZero(self.img_global),
NLinearInterpolatorFactory(),
# NearestNeighborInterpolatorFactory(),
),
transforms[o],
),
display_params[0],
display_params[1],
)
for o in self.serial_order
]
img_local = Views.permute(Views.addDimension(Views.stack(imgs), 0, 0), 3, 2)
if straight_transforms is None:
self.img_local = img_local
IL.show(self.img)
self.image_local = IJ.getImage()
else:
IL.show(img_local)
im = IJ.getImage()
im.hide()
return im
if DEBUG:
IJ.log(
"image local {} - {}".format(
self.image_local.getWidth(), self.image_local.getHeight()
)
)
def create_local_straight_stack(self):
straight_transforms = {}
for id_magc in self.sections:
t = AffineTransform2D()
t.translate(self.sections[id_magc].centroid)
t = t.inverse()
straight_transforms[id_magc] = t
im = self.create_local_stack(straight_transforms).getStack()
w, h = im.getWidth(), im.getHeight()
flattened_ims = []
for id_serial, id_section in enumerate(self.serial_order):
if id_section not in self.magnets:
continue
title = "section_magc_{:04}_serial_{:04}".format(id_section, id_serial)
im_p = im.getProcessor(id_serial + 1).duplicate()
flattened = ImagePlus("", im_p)
centroid_magnet = self.magnets[id_section].centroid
centroid_section = self.sections[id_section].centroid
roi_magnet = PointRoi(
centroid_magnet[0] - centroid_section[0] + 0.5 * w,
centroid_magnet[1] - centroid_section[1] + 0.5 * h,
)
roi_magnet.setHandleSize(2)
roi_magnet.setStrokeWidth(1)
flattened.setRoi(roi_magnet)
flattened = flattened.flatten()
flattened.setTitle(title)
bare = ImagePlus(title, im_p)
flattened_ims.append(flattened)
flattened_ims.append(bare)
flattened_stack = ImageStack(w, h)
for flattened in flattened_ims:
flattened_stack.addSlice(flattened.getTitle(), flattened.getProcessor())
im_flattened_stack = ImagePlus("straight_magnet", flattened_stack)
IJ.save(
im_flattened_stack, os.path.join(self.root, "overview_straight_magnet.tiff")
)
def add(self, annotation_type, poly, annotation_id):
"""
Adds an annotation to the wafer and returns it
annotation_id is a tuple for rois (section_id, roi_id)
and an int otherwise
"""
if annotation_type is AnnotationType.ROI:
section_id = annotation_id[0]
else:
section_id = annotation_id
if (
annotation_type is AnnotationType.SECTION
and annotation_id not in self.serial_order
# must use serial order, not self.sections
# because some workflows must handle serial_order changes themselves
# (e.g. renumber sections)
):
# appends to serial order only if new section
self.serial_order.append(section_id)
self.stage_order.append(section_id)
if self.mode is Mode.GLOBAL:
annotation = Annotation(annotation_type, poly, annotation_id)
else:
# transform to global coordinates when adding from local mode
annotation = Annotation(
annotation_type,
self.GC.transform_points_to_poly(
self.GC.poly_to_points(poly),
self.poly_transforms_inverse[section_id],
),
annotation_id,
)
if annotation_type is AnnotationType.ROI:
if self.rois.get(section_id) is None:
self.rois[section_id] = {}
self.rois[section_id][annotation_id[1]] = annotation
else:
getattr(self, annotation_type.name)[section_id] = annotation
return annotation
def add_section(self, poly, annotation_id):
return self.add(AnnotationType.SECTION, poly, annotation_id)
def add_roi(self, poly, annotation_id):
return self.add(AnnotationType.ROI, poly, annotation_id)
def add_magnet(self, poly, annotation_id):
return self.add(AnnotationType.MAGNET, poly, annotation_id)
def add_focus(self, poly, annotation_id):
return self.add(AnnotationType.FOCUS, poly, annotation_id)
def remove_current(self):
if self.mode is Mode.GLOBAL:
return
selected_indexes = self.manager.getSelectedIndexes()
if len(selected_indexes) != 1:
IJ.showMessage(
"Warning",
"To delete an annotation with [x], one and only one annotation"
" must be selected in blue in the annotation manager",
)
return
selected_poly = self.manager.getRoi(selected_indexes[0])
poly_name = selected_poly.getName()
annotation_type, section_id, annotation_id = type_id(poly_name)
self.save()
if annotation_type in {
AnnotationType.MAGNET,
AnnotationType.ROI,
AnnotationType.FOCUS,
}:
if get_OK("Delete {}?".format(poly_name)):
delete_selected_roi()
self.image.killRoi()
if annotation_type is AnnotationType.ROI:
del self.rois[section_id][annotation_id]
else:
del getattr(self, annotation_type.name)[annotation_id]
# select the section
self.manager.select(
get_roi_index_from_name(str(self.sections[section_id]))
)
elif annotation_type is AnnotationType.SECTION:
# deleting a sections also deletes the linked annotations (roi(s),focus,magnet)
section_id_manager = get_roi_index_from_name(str(self.sections[section_id]))
linked_annotations = []
message = ""
# build the message by screening all existing linked annotations
for type_ in [
AnnotationType.FOCUS,
AnnotationType.MAGNET,
]:
linked_annotation = getattr(self, type_.name).get(annotation_id)
if linked_annotation is not None:
message += "{}\n \n".format(str(linked_annotation))
linked_annotations.append(linked_annotation)
if section_id in self.rois:
for _, subroi in sorted(self.rois[section_id].iteritems()):
message += "{}\n \n".format(str(subroi))
linked_annotations.append(subroi)
message = "".join(
[
"Delete {}?".format(poly_name),
"\n \nIt will also delete\n \n" if message else "",
message,
]
)
if not get_OK(message):
return
self.image.killRoi()
# delete linked annotations in manager and in wafer
for linked_annotation in linked_annotations:
# delete in roimanager
index = get_roi_index_from_name(str(linked_annotation))
delete_roi_by_index(index)
# delete in wafer
annotation_type, section_id, annotation_id = type_id(
str(linked_annotation)
)
if annotation_type is AnnotationType.ROI:
del self.rois[section_id][annotation_id]
else:
del getattr(self, linked_annotation.type_.name)[section_id]
# delete section in manager
section_id_manager = get_roi_index_from_name(str(self.sections[section_id]))
delete_roi_by_index(section_id_manager)
# update the orders
del self.serial_order[self.serial_order.index(section_id)]
del self.stage_order[self.stage_order.index(section_id)]
del self.sections[section_id]
del self.transforms[section_id]
del self.poly_transforms[section_id]
del self.poly_transforms_inverse[section_id]
self.image.close()
self.manager.reset()
if len(self) == 0:
self.start_global_mode()
else:
self.start_local_mode()
# select the next section
if section_id_manager < self.manager.getCount():
set_roi_and_update_roi_manager(section_id_manager)
else:
set_roi_and_update_roi_manager(self.manager.getCount() - 1)
def init_images_global(self):
if self.image_path is None:
dlog("Warning: images not properly initialized")
return
self.image_global = IJ.openImage(self.image_path)
self.img_global = IL.wrap(self.image)
def arrange_windows(self):
IJ.getInstance().setLocation(
0, IJ.getScreenSize().height - IJ.getInstance().getSize().height
)
# enlarge window, select first ROI, get polygon tool
win = self.image.getWindow()
if self.image.getNSlices() > 1:
win.remove(win.getComponents()[1]) # remove slider bar
win.maximize()
win.setLocation(
IJ.getScreenSize().width
- self.manager.getSize().width
- win.getSize().width,
0,
)
# adjust focus
self.manager.toFront()
time.sleep(0.05)
IJ.selectWindow(self.image.getTitle())
def start_global_mode(self):
self.mode = Mode.GLOBAL
IJ.run("Labels...", "color=white font=10 use draw")
self.image.show()
self.arrange_windows()
self.wafer_to_manager()
self.set_listeners()
time.sleep(0.1)
self.manager.runCommand("Show All")
# self.manager.runCommand("Show All with labels")
IJ.run("Labels...", "color=white font=10 use draw")
self.manager.runCommand("Show All without labels")
def suggest_annotation_ids(self, annotation_type):
item_ids = [item.id_ for item in getattr(self, annotation_type.name).values()]
return suggest_ids(item_ids)
def get_closest(self, annotation_type, point):
"""Get the annotations of a certain type closest to a given point"""
distances = sorted(
[
[self.GC.get_distance(point, item.centroid), item]
for item in getattr(self, annotation_type.name).values()
]
)
return [d[1] for d in distances]
def get_display_parameters(self):
"""calculate [display_size, crop_size, tissue_magnet_distance] based on sectionSize"""
tissue_magnet_distance = 0
section_extent = 0
for section in self.sections.values()[: min(5, len(self))]:
section_extent = max(
section_extent, self.GC.longest_diagonal(section.points)
)
width = (
intr(DISPLAY_FACTOR * section_extent)
if not self.magnets
else intr(DISPLAY_FACTOR * 1.2 * section_extent)
)
width = width - width % 2
display_size = [width] * 2