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Water collection and filtration protocol using sterivex filters to collect envirnomental DNA from marine ecosystems. This collection and filtration protocol is used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
This PCR protocol is for amplifying This protocol is for amplifying the small subunit ribosomal ribonucleic acid (SSU rRNA) 18S v8 gene in eukaryotes.
Nucleic acids extraction from the sterivex filters using the Qiagen DNeasy Blood and Tissue Kit with some modifications to the manufacturer’s protocol. These extraction protocols, adapted from Spens et al 2017, are used by the NOAA PMEL Ocean Molecular Ecology (OME) Group.
This PCR protocol is for amplifying the 16S large subunit ribosomal RNA mitochondrial gene in eukaryotes.
This PCR protocol is for amplifying the Small subunit ribosomal ribonucleic acid (SSU rRNA) 18S v9 gene in eukaryotes.
This PCR protocol is for amplifying the mitochondrial displacement loop (D-loop) gene also known as the mitochondial control region in marine mammals.
This PCR protocol is for amplifying the 16S rRNA v4-v5 region.
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