diff --git a/group_vars/sn06.yml b/group_vars/sn06.yml
index 5ac37da52..13e372d0e 100644
--- a/group_vars/sn06.yml
+++ b/group_vars/sn06.yml
@@ -233,6 +233,7 @@ gie_proxy_nodejs_version: "10.13.0"
 gie_proxy_virtualenv: "{{ galaxy_root }}/gie-proxy/venv"
 gie_proxy_setup_service: systemd
 gie_proxy_sessions_path: "{{ galaxy_mutable_data_dir }}/interactivetools_map.sqlite"
+gie_proxy_path_prefix: /interactivetool/ep
 gie_proxy_port: 8800
 gie_proxy_verbose: true
 
diff --git a/group_vars/sn07.yml b/group_vars/sn07.yml
index 3eac16461..6acea1d96 100644
--- a/group_vars/sn07.yml
+++ b/group_vars/sn07.yml
@@ -287,6 +287,7 @@ gie_proxy_nodejs_version: "10.13.0"
 gie_proxy_virtualenv: "{{ galaxy_root }}/gie-proxy/venv"
 gie_proxy_setup_service: systemd
 gie_proxy_sessions_path: "{{ galaxy_mutable_data_dir }}/interactivetools_map.sqlite"
+gie_proxy_path_prefix: /interactivetool/ep
 gie_proxy_port: 8800
 gie_proxy_verbose: true
 
diff --git a/templates/nginx/galaxy-ie-main.j2 b/templates/nginx/galaxy-ie-main.j2
index 6205f276b..eb62a5827 100644
--- a/templates/nginx/galaxy-ie-main.j2
+++ b/templates/nginx/galaxy-ie-main.j2
@@ -17,7 +17,7 @@ server {
 	access_log /var/log/nginx/interactive.log;
 
 	location / {
-		proxy_pass http://127.0.0.1:8800;
+		proxy_pass http://127.0.0.1:{{ gie_proxy_port }};
 		proxy_redirect off;
 		proxy_http_version 1.1;
 		proxy_set_header Host $host;
diff --git a/templates/nginx/galaxy-main.j2 b/templates/nginx/galaxy-main.j2
index 75c2fc016..9fba5191c 100644
--- a/templates/nginx/galaxy-main.j2
+++ b/templates/nginx/galaxy-main.j2
@@ -181,6 +181,16 @@ server {
 		proxy_set_header Connection "upgrade";
 	}
 
+	# Route all path-based interactive tool requests to the InteractiveTool proxy application
+    location ~* ^/(interactivetool/.+)$ {
+        proxy_redirect off;
+        proxy_http_version 1.1;
+        proxy_set_header Host $host;
+        proxy_set_header X-Real-IP $remote_addr;
+        proxy_set_header Upgrade $http_upgrade;
+        proxy_set_header Connection "upgrade";
+        proxy_pass http://127.0.0.1:{{ gie_proxy_port }};
+    }
 
 	location /.well-known/ {
 		proxy_set_header           Host $host:$server_port;