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* Update README.md

Added text regarding the scANANSE publication & links

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Co-authored-by: Jos Smits <J.Smits@science.ru.nl>
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siebrenf and JGASmits authored Nov 15, 2023
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[![Maintainability](https://api.codeclimate.com/v1/badges/875df8c40fec66d68b1f/maintainability)](https://codeclimate.com/github/vanheeringen-lab/ANANSE/maintainability)
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### Prediction of key transcription factors in cell fate determination using enhancer networks
## Prediction of key transcription factors in cell fate determination using enhancer networks
ANANSE is a computational approach to infer enhancer-based gene regulatory networks (GRNs) and to identify key transcription factors between two GRNs. You can use it to study transcription regulation during development and differentiation, or to generate a shortlist of transcription factors for trans-differentiation experiments.

ANANSE is written in Python and comes with a command-line interface that includes 3 main commands: `ananse binding`, `ananse network`, and `ananse influence`. A graphical overview of the tools is shown below.
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For documentation on the **development version** see [here](https://anansepy.readthedocs.io/en/develop/).


## Citation

> ANANSE: an enhancer network-based computational approach for predicting key transcription factors in cell fate determination
> Quan Xu, Georgios Georgiou, Siebren Frölich, Maarten van der Sande, Gert Jan C Veenstra, Huiqing Zhou, Simon J van Heeringen
> Nucleic Acids Research, gkab598, https://doi.org/10.1093/nar/gkab598

## scANANSE: Gene regulatory network and motif analysis of single-cell clusters

scANANSE is a pipeline developed for single-cell RNA-sequencing data and single-cell ATAC-sequencing data. It can export single-cell cluster data from both Seurat or Scanpy objects, and runs the clusters through ANANSE using a snakemake workflow to significantly simplify the process. Afterwards, results can be imported back into your single-cell object.

For more info on this implementation check out the
* [scANANSE workflow](https://doi.org/10.12688/f1000research.130530.1)
* [Python package for Scanpy objects](https://github.com/Arts-of-coding/AnanseScanpy)
* [R package for Seurat objects](https://github.com/JGASmits/AnanseSeurat)
* [anansnake package for automating multiple ANANSE analyses](https://github.com/vanheeringen-lab/anansnake)


## Help and Support

* The preferred way to get support is through the [Github issues page](https://github.com/vanheeringen-lab/ANANSE/issues).


## License

- **[MIT license](http://opensource.org/licenses/mit-license.php)** [![Anaconda-Server Badge](https://anaconda.org/qxuchn/ananse/badges/license.svg)](https://anaconda.org/qxuchn/ananse)
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