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Merge pull request #3 from wanyuac/dev
Add support to ARIBA outputs
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| # Converting ARIBA outputs to an allelic PAM | ||
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| This subdirectory offers scripts for converting ARIBA outputs to an allelic PAM and a genetic PAM. Please see README under the main directory for a step-by-step guide. |
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| #!/usr/bin/env python | ||
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| """ | ||
| Converts output TSV of utility/tabulate_cdhit.py to an allelic presence-absence matrix (PAM) and saves | ||
| it in TSV format. | ||
| Command demonstration: | ||
| python clusters2pam.py -i alleles.clstr.tsv -om alleles_pam.tsv -ot alleles_clstr_updated.tsv | ||
| Dependency: module pandas, Python v3 | ||
| Copyright (C) 2020 Yu Wan <wanyuac@126.com> | ||
| Licensed under the GNU General Public Licence version 3 (GPLv3) <https://www.gnu.org/licenses/>. | ||
| Publication: 11 Nov 2020; the latest modification: 12 Nov 2020 | ||
| """ | ||
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| import os | ||
| import sys | ||
| import pandas | ||
| from argparse import ArgumentParser | ||
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| def parse_arguments(): | ||
| parser = ArgumentParser(description = "Creating an allelic presence-absence matrix from a table of sequence clusters",\ | ||
| epilog = "This is a helper script of R package GeneMates.") | ||
| parser.add_argument("-i", dest = "i", type = str, required = True, help = "Input FASTA files") | ||
| parser.add_argument("-om", dest = "om", type = str, required = False, default = "allelic_PAM.tsv", help = "Output presence-absence matrix in TSV format") | ||
| parser.add_argument("-ot", dest = "ot", type = str, required = False, default = "clusters.tsv", help = "Output table about sequence clusters") | ||
| return parser.parse_args() | ||
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| def main(): | ||
| args = parse_arguments() | ||
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| # Import the input TSV file as a data frame | ||
| input_tsv = args.i | ||
| if os.path.exists(input_tsv): | ||
| clusters = parse_seqid(pandas.read_csv(input_tsv, sep = "\t", index_col = None)) # Import the table as a data frame of six columns and parse column 'seqid' | ||
| else: | ||
| print("Error: the input file is not accessible.", file = sys.stderr) | ||
| sys.exit(1) | ||
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| # Assign allele IDs and create an allelic PAM from data frame "clusters" | ||
| pam = create_allelic_pam(clusters) | ||
| pam.to_csv(args.om, header = True, index = False, sep = "\t", float_format = None) | ||
| clusters.to_csv(args.ot, header = True, index = False, sep = "\t", float_format = None) | ||
| return | ||
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| def parse_seqid(df): | ||
| """ | ||
| Parses column 'seqid' into two new columns and returns a data frame of eight columns. | ||
| """ | ||
| seqids = df["seqid"].tolist() # Convert a column into a list | ||
| genes = list() | ||
| samples = list() | ||
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| # Create two lists from one column | ||
| for item in seqids: | ||
| g, s = item.split("|") | ||
| genes.append(g) | ||
| samples.append(s) | ||
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| # Append lists to the data frame as columns | ||
| df["gene"] = genes | ||
| df["sample"] = samples | ||
| return df | ||
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| def create_allelic_pam(df): | ||
| """ | ||
| Assign allele IDs and create an allelic PAM based on clustering results. | ||
| """ | ||
| samples = get_unique_ids(df, "sample") | ||
| cluster_ids = get_unique_ids(df, "cluster") # 0, 1, 2, ... | ||
| genes_visited = dict() # A dictionary of genes in processed clusters. {gene : number of clusters} | ||
| pam = pandas.DataFrame(samples, columns = ["sample"]) # Initalise the output PAM | ||
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| # Create a list about presence-absence of each allele and combine it to the output PAM as a column | ||
| for c in cluster_ids: # Type of elements: numpy.int64 | ||
| df_c = df[df["cluster"] == c] # Select rows of the current cluster | ||
| genes_c = df_c["gene"].tolist() # All gene names should be the same when alleles are clustered under 100% nucleotide identity | ||
| gene = genes_c[0] | ||
| if gene in genes_visited.keys(): | ||
| genes_visited[gene] += 1 | ||
| allele = gene + "." + str(genes_visited[gene]) # Adding a suffix for making an allele name. Example result: sul1.1, sul1.2. | ||
| else: | ||
| genes_visited[gene] = 0 # Record a new gene encountered | ||
| allele = gene | ||
| pa_vec = list() # A binary vector about presence-absence of the current allele across samples | ||
| samples_c = df_c["sample"].tolist() # Samples in which the current allele is detected | ||
| for s in samples: | ||
| pa = 1 if s in samples_c else 0 | ||
| pa_vec.append(pa) | ||
| pam[allele] = pa_vec | ||
| return pam | ||
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| def get_unique_ids(df, col_name): | ||
| """ | ||
| A subordinate function of create_allelic_pam for getting a list of unique and sorted values from a | ||
| given column of input data frame df. | ||
| """ | ||
| ids = list(df[col_name].unique()) | ||
| ids.sort(reverse = False) | ||
| return ids | ||
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| if __name__ == "__main__": | ||
| main() |
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| #!/usr/bin/env python | ||
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| """ | ||
| Pool FASTA files of ARIBA's output allele sequences into one file and append sample names to sequence IDs (in | ||
| the same format as that for SRST2's output consensus sequences). | ||
| Command demonstration: | ||
| python pool_seqs.py -i *_genes.fna -o alleles.fna -e '_genes.fna' | ||
| Copyright (C) 2020 Yu Wan <wanyuac@126.com> | ||
| Licensed under the GNU General Public Licence version 3 (GPLv3) <https://www.gnu.org/licenses/>. | ||
| Publication: 11 Nov 2020; the latest modification: 11 Nov 2020 | ||
| """ | ||
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| import os | ||
| from argparse import ArgumentParser | ||
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| def parse_arguments(): | ||
| parser = ArgumentParser(description = "Pool ARIBA's output allele sequences into one FASTA file and append sample names to sequence IDs",\ | ||
| epilog = "This is a helper script of R package GeneMates.") | ||
| parser.add_argument("-i", nargs = "+", dest = "i", type = str, required = True, help = "Input FASTA files") | ||
| parser.add_argument("-o", dest = "o", type = str, required = False, default = "alleles.fna", help = "Output FASTA file of pooled allele sequences") | ||
| parser.add_argument("-e", dest = "e", type = str, required = False, default = "_genes.fna", help = "Filename extension to be removed for sample names") | ||
| return parser.parse_args() | ||
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| def main(): | ||
| args = parse_arguments() | ||
| fasta_out = open(args.o, "w") | ||
| sample_count = 0 | ||
| for fasta_in in args.i: | ||
| f_in = open(fasta_in, "r") | ||
| fasta_in = os.path.basename(fasta_in) | ||
| sample = fasta_in.replace(args.e, "") # No change applies if args.e is not found in the filename | ||
| line = f_in.readline() | ||
| while line: | ||
| if line.startswith(">"): # A header is encountered | ||
| fields = line.split(".") # ARIBA uses full stops as delimiters in the header line | ||
| new_id = fields[0] + "|" + sample # Example value: ">cluster_1|sample_1" | ||
| seq_descr = ".".join(fields[1 : ]) | ||
| fasta_out.write(new_id + " " + seq_descr) # Note that the newline character of this line is not stripped off by the readline method. | ||
| else: | ||
| fasta_out.write(line) | ||
| line = f_in.readline() # Till the end of the file, a None value is returned. | ||
| f_in.close() | ||
| sample_count += 1 | ||
| fasta_out.close() | ||
| print("%d samples have been processed." % sample_count) | ||
| return | ||
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| if __name__ == "__main__": | ||
| main() |