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Welcome to the sequencing_center wiki!

Linux system problem.

• Docker images
• MySQL command
• Linux command
• How to backup
• How to create a database
• CPU Memery
• How to mount
• Linux add user
• auto password
• 修改内网静态IP
• Ubuntu ssh
• Ubuntu build-essentia
• Ubuntu16.04挂载新硬盘并格式化硬盘
• Burn iso into flash disk
• bash loop if
• merge file
• md5sum
• function paramter
• bash string split
• bash replace string
• bash cut sort
• bash dynamic (variable) variable names
• Docker

Bioinformatic languages

• genome annotation

Pipeline

• xtail
• correlation
• edgeR
• stouffer
• PCA
• blast
• star genome

R system

• R module
• R demo
• R file path
• R order dataframe by column.
• r install
• R old version install bioconductor install R packages.
• apply method
• R function
• barplot without border and color
• r paste
• volcano plot
• R save RData

python

• conda env
• module.py

awk

perl system

• perl function
• max
• perl split join
• perl for loop
• Perl substring
• perl variable as variable
• perl: add elements to an array

Database

• search

Tensorflow

• Install in windows10
• Learning demo

Nanopore technology

fast5_formate
Basecalling with Guppy
adapter
Data Quality Assessment
nanopore map
nanopore assembly
Annotating the genome with prokka
h5py
fast5mod
megalodon

circRNA

• CIRCexplorer2
• find_circ
• CIRI

Website

• web basic
• Disable browse web file
• xixiaochendata
• Move hub database to 27
• image to svg
• venn

RPKM, FPKM and TPM, clearly explained